Restriction Digest Analysis
8:02
YouTubeAddgene
Restriction Digest Analysis
In this video, we will digest a plasmid of known identity with restriction enzymes, run the products on an agarose gel, and analyze the results. We’ll focus on identifying the bands produced under different conditions and use the results to make conclusions about the plasmid. Note: XbaI is effected by Dam methylation - see this blog post for ...
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Restriction Digest Tutorial
Restriction Digest of Plasmid DNA
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[GET ANSWER] 7: 56 ⋯Δ(1) 4#1 Mol Bio Re...Complete the following problems.Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by
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