Immunoprecipitation Techniques: Purification of Proteins
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Calculations for the Purification of a Recombinant Protein The purification of a recombinant protein is carried out starting with 100 liters of a clarified cell lysate (i.e., the cells have been lysed, and the cell debris has been removed to give a clarified solution), which has a total protein concentration of 0.36 mg / ml and a recombinant protein concentration of 2.2 U / ml, where U denotes units of biological activity of the recombinant protein. It is known that the completely pure recombina
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